Cor Vasa 2025, 67(3):360-366 | DOI: 10.33678/cor.2025.004

Correlation of ITGB3 Gene rs5918T>C and APOA1 Gene rs1799837C>T Variants with Serum Lipid Profiles in Turkish Cypriot Patients with Coronary Artery Disease

Cenk Conkbayir^a, Mahmut Cerkez Ergoren^{b, c}, Havva Cobanogullari^{b, c}, Ozlem Balcioglu^d, Irem Abras^e, Tengul Eminsel^e, Didem Melis Oztas^f, Burak Onal^g, Murat Ugurlucan^f, Sehime Gulsun Temel^{h, i}

^a Department of Cardiology, Faculty of Medicine, Near East University, Nicosia, Cyprus

^b Department of Medical Biology, Faculty of Medicine, Near East University, Nicosia, Cyprus

^c DESAM Institute, Near East University, Nicosia, Cyprus

^d Department of Cardiovascular Surgery, Faculty of Medicine, Near East University, Nicosia, Cyprus

^e Department of Biological Sciences, Eastern Mediterranean University, Famagusta, Cyprus

^f Department of Cardiovascular Surgery Clinic, Biruni University Faculty of Medicine, Istanbul, Turkey

^g Department of Medical Pharmacology, Biruni University Faculty of Medicine, Istanbul, Turkey

^h Department of Medical Genetics, Faculty of Medicine, Uludag University, Bursa, Turkey

ⁱ Department of Histology and Embryology, Faculty of Medicine, Uludag University, Bursa, Turkey

Aim: The relationship between hypercholesterolemia, particularly elevated low density lipoprotein-cholesterol (LDL-C) levels and coronary artery disease is recognized by the evidence from previous epidemiologic studies. Importantly, genetic polymorphisms on different genes have been reported to be associated with plasma lipid levels. In this particular study, we aimed to investigate the relationship between the ITGB3 gene rs5918 T>C and APO-A1 gene rs1799837 C>T markers and serum lipid metabolism.

Patients and methods: A total of 100 subjects with CAD and 250 healthy subjects were involved in the current study. A basic biochemical analysis, including serum glucose, total serum cholesterol, HDL-C, LDL-C and triglycerides, was performed for each participant. Genotyping for the ITGB3 gene and APO-A1 gene polymorphisms was performed by polymerase chain reaction followed by restriction fragment length polymorphism (RFLP) analysis.

Results: With respect to the genotype and allele distributions of ITGB3 rs5918 T>C polymorphism, the frequency of the C allele was higher in the coronary artery disease (CAD) group compared to the control group (p = 0.001). Moreover, there was a statistically significant association detected between ITGB3 rs5918 CC genotype and serum total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) (p = 0.0006, p = 0.016, respectively) in CAD group. However there was no statistically significant association was identified between the APOA1 rs1799837 C>T polymorphism and biochemical parameters in control and CAD group.

Conclusion: The results demonstrated that rs5918 T>C variant within the ITGB3 gene might have a clinical importance as a genetic marker which increases the susceptibility to CAD. Therefore, the ITGB3 gene rs5918 C allele may be offered as a screening option for CAD in Turkish Cypriot population who come in for medical check-up.

Keywords: , APO-A1, Coronary artery disease, HDL-C, ITGB3, LDL-C

Received: October 30, 2024; Revised: December 18, 2024; Accepted: January 4, 2025; Prepublished online: June 2, 2012; Published: June 20, 2025  Show citation