Cor Vasa 2024, 66(6):587-591 | DOI: 10.33678/cor.2024.059

The Effect of Lisinopril on Angiogenesis of Endothelial Progenitor Cells (EPCs) in Stable Coronary Heart Disease Post COVID-19

Yudi Her Oktavionoa, Mohammad Budiartoa, Johannes Nugroho Eko Putrantoa, Budi Susetyo Pikira, Andriantoa, Meity Ardianaa, Makhyan Jibril Al-Farabib, Dara Ninggar Ghassania, Bagus Putra Dharma Khrisnaa, Sondang Jasmine Mustikasari Sitorusa, Maltadila Ratu Hajjrina, Pandit Bagus Tri Saputraa
a Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
b University College London, London, United Kingdom

Background: COVID-19 infection is associated with endothelial dysfunction. The concept of endothelial cell repair utilizing endothelial progenitor cells (EPCs) with the use of angiotensin-converting enzyme inhibitors (ACEIs) has been developing which is known for its potential for EPC's angiogenesis improvement.

Methods: This is a true experimental in vitro study with post-test only control group design. Mononuclear cells were isolated from peripheral venous blood of patient with chronic coronary syndrome and history of COVID-19. The cells then cultured on special media for 7 days and EPC was identified using immunocytochemical examination with labelled anti-CD34 cells FITC-under fluorescence microscope examination. The cells were divided into two groups consisted of control group and 50 μM lisinopril-treated group. The morphology and tube connections were analyzed using Wimasis image analyzer. The von Willebrand factor (vWF) and CD31 concentrations were also measured by ELISA. Statistical comparison between both groups was performed and p-value < 0.05 was considered significant.

Keywords: Angiogenesis, Coronary heart disease, COVID-19, Endothelial progenitor cells, Lisinopril, Tube formation,

Received: July 7, 2024; Revised: July 7, 2024; Accepted: July 28, 2024; Prepublished online: June 2, 2012; Published: December 20, 2024  Show citation

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Her Oktaviono Y, Budiarto M, Eko Putranto JN, Pikir BS, Andrianto, Ardiana M, et al.. The Effect of Lisinopril on Angiogenesis of Endothelial Progenitor Cells (EPCs) in Stable Coronary Heart Disease Post COVID-19. Cor Vasa. 2024;66(6):587-591. doi: 10.33678/cor.2024.059.
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