Cor Vasa 2022, 64(6):589-594 | DOI: 10.33678/cor.2022.033

Association between reversine dose and increased plasticity of dedifferentiated fat (DFAT cells) into cardiac derived cells

Muhammad Firdani Ramadhan, Yudi Her Oktaviono, Budi Baktijasa Dharmadjati, Ryan Enast Intan

Department of Cardiology and Vascular Medicine, Faculty of Medicine Universitas Airlangga - Dr. Soetomo General Hospital, Surabaya, East Java, Indonesia

Aim: To analyze the association between reversine and increased plasticity of DFAT into cardiac derivative cells.

Method: The cultured DFAT cells were divided into four groups based on reversine dose: control (no reversine), 10 nM, 20 nM, and 40 nM reversine. Each group will go through several stages of passage before further differentiation into cardiomyocytes (marked by cTnT expression), VSCMs (marked by alpha-SMA expression), and vascular endothelial cells (marked by alpha-SMA expression) (marked by CD31 expression).

Result: There were significant differences in the expression of cTnT, alpha-SMA, and CD31 (p = 0.003, p <0.001, and p <0.001, respectively) in each group of DFAT cells that received reversine. From post-hoc analysis with Tukey test, it was found that only the 10 nM reversine group produced a significant difference compared to the control group (p = 0.002) for cTnT expression and reversine 10 nM and 20 nM group for α-SMA expression and CD31 expression (p = 0.028 and p <0.001, respectively).

Conclusions: This study proves that there is a relationship between reversine and increased plasticity of DFAT cells into cardiac derived cells in the form of cardiomyocytes (cTnT), VSMCs (alpha-SMA), and vascular endothelial cells (CD31).

Keywords: Cardiomyocyte, DFAT, Reversine, Vascular endothelial cells, VSMCs

Received: March 6, 2022; Revised: March 6, 2022; Accepted: April 3, 2022; Published: December 15, 2022  Show citation